STX8
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
STX7
Gene Ontology Biological Process
- intracellular protein transport [IBA]
- organelle assembly [IDA]
- organelle localization [IDA]
- positive regulation of T cell mediated cytotoxicity [IMP]
- positive regulation of receptor localization to synapse [IMP]
- regulation of protein localization to plasma membrane [IDA]
- vesicle docking [IBA]
- vesicle fusion [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IBA]
- azurophil granule [IDA]
- early endosome [IDA]
- endocytic vesicle [IDA]
- endomembrane system [IBA]
- endosome [IDA]
- extracellular vesicular exosome [IDA]
- immunological synapse [IDA]
- integral component of membrane [IBA]
- intracellular membrane-bounded organelle [IDA]
- late endosome [IDA]
- lysosomal membrane [IDA]
- lysosome [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tertiary granule [IDA]
- vesicle [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function.
Sets of SNARE proteins mediate membrane fusion by assembling into core complexes. Multiple SNAREs are thought to function in different intracellular trafficking steps but it is often unclear which of the SNAREs cooperate in individual fusion reactions. We report that syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8 form a complex that functions in the fusion of late endosomes. Antibodies specific ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STX7 STX8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3373015 | |
| STX7 STX8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9999 | BioGRID | 1177648 | |
| STX7 STX8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2225281 | |
| STX8 STX7 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3054258 | |
| STX7 STX8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| STX7 STX8 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 100 | BioGRID | 3008523 |
Curated By
- BioGRID