BAIT

RGS4

RGP4, SCZD9, RP11-430G6.1
regulator of G-protein signaling 4
GO Process (2)
GO Function (2)
GO Component (2)
Homo sapiens
PREY

COPB2

beta'-COP
coatomer protein complex, subunit beta 2 (beta prime)
GO Process (4)
GO Function (0)
GO Component (2)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

RGS4 and RGS2 bind coatomer and inhibit COPI association with Golgi membranes and intracellular transport.

Sullivan BM, Harrison-Lavoie KJ, Marshansky V, Lin HY, Kehrl JH, Ausiello DA, Brown D, Druey KM

COPI, a protein complex consisting of coatomer and the small GTPase ARF1, is an integral component of some intracellular transport carriers. The association of COPI with secretory membranes has been implicated in the maintenance of Golgi integrity and the normal functioning of intracellular transport in eukaryotes. The regulator of G protein signaling, RGS4, interacted with the COPI subunit beta'-COP in ... [more]

Mol. Biol. Cell Sep. 01, 2000; 11(9);3155-68 [Pubmed: 10982407]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
COPB2 RGS4
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
RGS4 COPB2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID