Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interaction of Deubiquitinase 2A-DUB/MYSM1 with DNA Repair and Replication Factors.

Kroeger C, Roesler R, Wiese S, Hainzl A, Gatzka MV

The deubiquitination of histone H2A on lysine 119 by 2A-DUB/MYSM1, BAP1, USP16, and other enzymes is required for key cellular processes, including transcriptional activation, apoptosis, and cell cycle control, during normal hematopoiesis and tissue development, and in tumor cells. Based on our finding that MYSM1 colocalizes with ?H2AX foci in human peripheral blood mononuclear cells, leukemia cells, and melanoma cells ... [more]

Int J Mol Sci May. 26, 2020; 21(11); [Pubmed: 32466590]

Throughput

  • High Throughput

Additional Notes

  • assayed using SCX-HPLC MS/MS (tandem mass spectrometry)

Curated By

  • BioGRID