BAIT

DTX3L

BBAP
deltex 3 like, E3 ubiquitin ligase
UPS Project
GO Process (3)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

EPB41L2

4.1-G, 4.1G, RP3-324N14.1
erythrocyte membrane protein band 4.1-like 2
GO Process (0)
GO Function (0)
GO Component (8)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

DELTEX2 C-terminal domain recognizes and recruits ADP-ribosylated proteins for ubiquitination.

Ahmed SF, Buetow L, Gabrielsen M, Lilla S, Chatrin C, Sibbet GJ, Zanivan S, Huang DT

Cross-talk between ubiquitination and ADP-ribosylation regulates spatiotemporal recruitment of key players in many signaling pathways. The DELTEX family ubiquitin ligases (DTX1 to DTX4 and DTX3L) are characterized by a RING domain followed by a C-terminal domain (DTC) of hitherto unknown function. Here, we use two label-free mass spectrometry techniques to investigate the interactome and ubiquitinated substrates of human DTX2 and ... [more]

Sci Adv Aug. 01, 2020; 6(34); [Pubmed: 32937373]

Throughput

  • High Throughput

Additional Notes

  • proteins identified as binding partners from mass spectrometric analysis of co-immunoprecipited proteins if T-test difference was less than -2.61 and negative log of T-test p-value was greater than 1.33

Curated By

  • BioGRID