Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

DBC1, p300, HDAC3, and Siah1 coordinately regulate ELL stability and function for expression of its target genes.

Basu S, Barad M, Yadav D, Nandy A, Mukherjee B, Sarkar J, Chakrabarti P, Mukhopadhyay S, Biswas D

Among all of the Super Elongation Complex (SEC) components, ELL1 (also known as ELL) is the only bona fide elongation factor that directly stimulates transcription elongation by RNA polymerase II. However, the mechanism(s) of functional regulation of ELL1 (referred to as ELL hereafter), through its stabilization, is completely unknown. Here, we report a function of human DBC1 in regulating ELL ... [more]

Proc Natl Acad Sci U S A Dec. 24, 2019; 117(12);6509-6520 [Pubmed: 32152128]

Throughput

  • High Throughput

Curated By

  • BioGRID