MT3
Gene Ontology Biological Process
- astrocyte development [IDA]
- cadmium ion homeostasis [IMP]
- cellular lipid catabolic process [IMP]
- cellular metal ion homeostasis [IDA]
- cellular response to oxidative stress [IDA]
- cellular zinc ion homeostasis [IDA, IMP]
- cholesterol catabolic process [IMP]
- energy reserve metabolic process [IMP]
- leptin-mediated signaling pathway [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of autophagy [IMP]
- negative regulation of necrotic cell death [IMP]
- negative regulation of neurogenesis [IDA]
- negative regulation of neuron apoptotic process [IDA]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of catalytic activity [IMP]
- positive regulation of cell death [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of lysosomal membrane permeability [IMP]
- positive regulation of oxygen metabolic process [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of protein glycosylation [IMP]
- regulation of response to food [IMP]
- response to oxidative stress [IMP]
- zinc ion transport [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ACTB
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- NuA4 histone acetyltransferase complex [ISO]
- axon [ISO]
- blood microparticle [ISO]
- cortical cytoskeleton [IDA]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosol [IDA]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [IDA]
- nuclear chromatin [ISO]
- postsynaptic density [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [ISO]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of mouse brain proteins associated with isoform 3 of metallothionein.
Using immunological approaches and mass spectrometry, five proteins associated with metallothionein-3 in mouse brains have been identified. Metallothionein-3 and associated proteins were isolated using immunoaffinity chromatography over immobilized anti-mouse brain MT3 antibody. Proteins in the recovered pool were separated by SDS-polyacrylamide gel electrophoresis, and distinct bands were excised and the proteins digested using trypsin. Peptides were extracted and analyzed using ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
MT3 ACTB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
MT3 ACTB | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID