BAIT

DNMT1

DNA MeTase, xDnmt1
DNA (cytosine-5-)-methyltransferase 1
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis
PREY

HIRA

dgcr1, hira-A, tup1, tuple1
histone cell cycle regulator
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation.

Nishiyama A, Mulholland CB, Bultmann S, Kori S, Endo A, Saeki Y, Qin W, Trummer C, Chiba Y, Yokoyama H, Kumamoto S, Kawakami T, Hojo H, Nagae G, Aburatani H, Tanaka K, Arita K, Leonhardt H, Nakanishi M

Stable inheritance of DNA methylation is critical for maintaining differentiated phenotypes in multicellular organisms. We have recently identified dual mono-ubiquitylation of histone H3 (H3Ub2) by UHRF1 as an essential mechanism to recruit DNMT1 to chromatin. Here, we show that PCNA-associated factor 15 (PAF15) undergoes UHRF1-dependent dual mono-ubiquitylation (PAF15Ub2) on chromatin in a DNA replication-coupled manner. This event will, in turn, ... [more]

Nat Commun Dec. 06, 2019; 11(1);1222 [Pubmed: 32144273]

Throughput

  • High Throughput

Curated By

  • BioGRID