An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Comprehensive analysis of the host-virus interactome of SARS-CoV-2

Chen Z, Wang C, Feng X, Nie L, Tang M, Zhang H, Xiong Y, Swisher SK, Srivastava M, Chen J

Host-virus protein-protein interaction is the key component of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lifecycle. We conducted a comprehensive interactome study between the virus and host cells using tandem affinity purification and proximity labeling strategies and identified 437 human proteins as the high-confidence interacting proteins. Functional characterization and further validation of these interactions elucidated how distinct SARS-CoV-2 viral ... [more]

Status: Preliminary Report

Throughput

High Throughput

Additional Notes

SFB-TAP: Tandem affinity purification (TAP) with the SFB (S-protein, FLAG epitope, and streptavidin-binding peptide) tag
The identified prey proteins were filtered using Significance Analysis of INTeractome (SAINTexpress) and those with a Bayesian false discovery rate of 0.05 or less were considered high-confidence interacting proteins (HCIPs).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NSP6 ATP5J	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu X (2021)	High	1	BioGRID	3209459

Curated By

BioGRID

Interaction Summary

NSP6

ATP5J

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IDA]transmembrane transporter activity [IC]transporter activity [NAS]

Gene Ontology Cellular Component

Affinity Capture-MS