Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Silencing of EphA3 through a cis interaction with ephrinA5.

Carvalho RF, Beutler M, Marler KJ, Knoell B, Becker-Barroso E, Heintzmann R, Ng T, Drescher U

EphAs and ephrinAs are expressed in multiple areas of the developing brain in overlapping countergradients, notably in the retina and tectum. Here they are involved in targeting retinal axons to their correct topographic position in the tectum. We have used truncated versions of EphA3, single-amino acid point mutants of ephrinA5 and fluorescence resonance energy transfer technology to uncover a cis ... [more]

Nat Neurosci Mar. 01, 2006; 9(3);322-30 [Pubmed: 16491080]

Throughput

  • Low Throughput

Curated By

  • BioGRID