BAIT

MDM2

ACTFS, HDMX, hdm2
MDM2 proto-oncogene, E3 ubiquitin protein ligase
GO Process (25)
GO Function (7)
GO Component (9)
Homo sapiens
PREY

SCN3B

ATFB16, BRGDA7, HSA243396, SCNB3
sodium channel, voltage-gated, type III, beta subunit
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Voltage-gated sodium channels ?3 subunit promotes tumorigenesis in hepatocellular carcinoma by facilitating p53 degradation.

Li S, Han J, Guo G, Sun Y, Zhang T, Zhao M, Xu Y, Cui Y, Liu Y, Zhang J

The voltage-gated sodium channels (VGSCs) are aberrantly expressed in a variety of tumors and play an important role in tumor growth and metastasis. Here, we show that VGSCs auxiliary ?3 subunit, encoded by the SCN3B gene, promotes proliferation and suppresses apoptosis in HepG2 cells by promoting p53 degradation. ?3 significantly increases HepG2 cell proliferation, promotes tumor growth in mouse xenograft ... [more]

FEBS Lett Dec. 01, 2019; 594(3);497-508 [Pubmed: 31626714]

Throughput

  • Low Throughput

Curated By

  • BioGRID