Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

JNK mediates insulin-like growth factor binding protein 2/integrin alpha5-dependent glioma cell migration.

Mendes KN, Wang GK, Fuller GN, Zhang W

We have previously shown that a molecular interaction between insulin-like growth factor binding protein 2 (IGFBP2) and integrin alpha5 is necessary for the enhancement of cell migration in IGFBP2-overexpressing gliomas. In the present study, we examined the mechanism through which the IGFBP2/integrin alpha5 interaction mediates enhanced glioma cell migration. Although both ERK and JNK MAP kinases were activated, JNK was ... [more]

Int J Oncol Jul. 01, 2010; 37(1);143-53 [Pubmed: 20514406]

Throughput

  • Low Throughput

Curated By

  • BioGRID