An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, Williams NS, Nijhawan D

Indisulam is an aryl sulfonamide drug with selective anticancer activity. Its mechanism of action and the basis for its selectivity have so far been unknown. Here we show that indisulam promotes the recruitment of RBM39 (RNA binding motif protein 39) to the CUL4-DCAF15 E3 ubiquitin ligase, leading to RBM39 polyubiquitination and proteasomal degradation. Mutations in RBM39 that prevent its recruitment ... [more]

Science Dec. 28, 2016; 356(6336); [Pubmed: 28302793]

Throughput

High Throughput

Additional Notes

Proteins that associated with RBM39 (control with no indisulam treatment). Contaminants were removed.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HSPB1 RBM39	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Katsogiannou M (2014)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RBM39

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HSPB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase C binding [ISS]protein kinase C inhibitor activity [ISS]protein kinase binding [IPI]ubiquitin binding [ISS]