PREY

NOP56

SIK1, snoRNP complex protein NOP56, L000002982, YLR197W
Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
GO Process (2)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome.

Lau B, Cheng J, Flemming D, La Venuta G, Berninghausen O, Beckmann R, Hurt E

Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension at the 18S rRNA that is integrated into the huge 90S pre-ribosome structure. Upon endo-nucleolytic cleavage at an internal site, ... [more]

Mol Cell Dec. 21, 2020; 81(2);293-303.e4 [Pubmed: 33326748]

Throughput

  • Low Throughput

Additional Notes

  • 90S pre-ribosomal particles were obtained by split-tag affinity purification using Csl4 as first bait and Dim1 as second bait.
  • 90S-exosome super-complex (state Post-A1-exosome)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NOP56 CSL4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High5BioGRID
3594835
CSL4 NOP56
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4559BioGRID
1949869

Curated By

  • BioGRID