Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Glutamate receptor subunit 3 is modified by site-specific limited proteolysis including cleavage by gamma-secretase.

Meyer EL, Strutz N, Gahring LC, Rogers SW

Ionotropic glutamate receptor (GluR) expression and function is regulated through multiple pre- and post-translational mechanisms. We find that limited proteolytic cleavage of GluR3 at two distinct sites generates stable GluR3 short forms that are glycosylated and found in association with other full-length GluRs in the mouse brain and cultured primary neurons. A combination of mutagenesis and transfection into HEK293 cells ... [more]

J Biol Chem Jun. 27, 2003; 278(26);23786-96 [Pubmed: 12700243]

Throughput

  • Low Throughput

Curated By

  • BioGRID