BAIT

ULK1

AU041434, Unc51.1, mKIAA0722
unc-51 like kinase 1
Mus musculus
PREY

MTOR

2610315D21Rik, AI327068, FRAP, FRAP2, Frap1, RAFT1, RAPT1, flat, RP23-423L9.1
mechanistic target of rapamycin (serine/threonine kinase)
GO Process (41)
GO Function (11)
GO Component (13)

Gene Ontology Biological Process

Mus musculus

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease.

Franco-Iborra S, Plaza-Zabala A, Montpeyo M, Sebastian D, Vila M, Martinez-Vicente M

The precise degradation of dysfunctional mitochondria by mitophagy is essential for maintaining neuronal homeostasis. HTT (huntingtin) can interact with numerous other proteins and thereby perform multiple biological functions within the cell. In this study, we investigated the role of HTT during mitophagy and analyzed the impact of the expansion of its polyglutamine (polyQ) tract. HTT is involved in different mitophagy ... [more]

Autophagy Feb. 24, 2020; ();1-18 [Pubmed: 32093570]

Throughput

  • Low Throughput

Additional Notes

  • Proximity Ligation Assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MTOR ULK1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID