GULP1
Gene Ontology Biological Process
CLTC
Gene Ontology Biological Process
- Golgi organization [IMP]
- mitotic nuclear division [IMP, ISO]
- negative regulation of hyaluronan biosynthetic process [ISO, ISS]
- negative regulation of protein localization to plasma membrane [ISO]
- osteoblast differentiation [ISO]
- receptor internalization [ISO]
- receptor-mediated endocytosis [IMP, ISO]
- transferrin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- clathrin coat [IDA, TAS]
- clathrin complex [ISO]
- clathrin-coated endocytic vesicle membrane [TAS]
- clathrin-coated vesicle [ISO]
- cytoplasm [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- membrane coat [ISO]
- mitochondrion [ISO]
- myelin sheath [ISO]
- protein complex [ISO]
- sarcolemma [IDA]
- spindle [IDA, ISO]
- terminal bouton [IDA]
- vesicle [ISO]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
A rat homologue of CED-6 is expressed in neurons and interacts with clathrin.
We isolated from a brain library a cDNA encoding an isoform of rat CED-6 that has not been previously described. This transcript results from alternative splicing of the ced-6 gene present on chromosome 9. We expressed this isoform as his-tagged protein in E. coli and used the purified protein to raise antibodies to investigate the expression of CED-6 in rat ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GULP1 CLTC | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID