BAIT

CRD1

CLS1, cardiolipin synthase, L000003358, YDL142C
Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

CIT2

citrate (Si)-synthase CIT2, L000000342, YCR005C
Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Cardiolipin-deficient cells depend on anaplerotic pathways to ameliorate defective TCA cycle function.

Raja V, Salsaa M, Joshi AS, Li Y, van Roermund CWT, Saadat N, Lazcano P, Schmidtke M, Huettemann M, Gupta SV, Wanders RJA, Greenberg ML

Previous studies have shown that the cardiolipin (CL)-deficient yeast mutant, crd1?, has decreased levels of acetyl-CoA and decreased activities of the TCA cycle enzymes aconitase and succinate dehydrogenase. These biochemical phenotypes are expected to lead to defective TCA cycle function. In this study, we report that signaling and anaplerotic metabolic pathways that supplement defects in the TCA cycle are essential ... [more]

Biochim Biophys Acta Mol Cell Biol Lipids Dec. 01, 2018; 1864(5);654-661 [Pubmed: 30731133]

Throughput

  • Low Throughput

Ontology Terms

  • inviable (APO:0000112)
  • heat sensitivity (APO:0000147)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CIT2 CRD1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.0848BioGRID
535278

Curated By

  • BioGRID