BAIT

RNF40

BRE1B, RBP95, STARING
ring finger protein 40, E3 ubiquitin protein ligase
UPS Project
Homo sapiens
PREY

EEF1D

EF-1D, EF1D, FP1047, UNQ601
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Homo sapiens

Biochemical Activity (Ubiquitination)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

RNF20/40-mediated eEF1B?L monoubiquitylation stimulates transcription of heat shock-responsive genes.

In S, Kim YI, Lee JE, Kim J

RNF20/40 E3 ubiquitin ligase-mediated histone H2B monoubiquitylation plays important roles in many cellular processes, including transcriptional regulation. However, the multiple defects observed in RNF20-depleted cells suggest additional ubiquitylation targets of RNF20/40 beyond histone H2B. Here, using biochemically defined assays employing purified factors and cell-based analyses, we demonstrate that RNF20/40, in conjunction with its cognate E2 ubiquitin-conjugating enzyme RAD6, monoubiquitylates lysine ... [more]

Nucleic Acids Res. Dec. 08, 2018; 47(6);2840-2855 [Pubmed: 30649429]

Throughput

  • Low Throughput

Additional Notes

  • in vitro ubiquitination with Ube1 as E1, Rad6A as E2, RNF20 and RNF40 as E3 and eEF1BdeltaL as substrate
  • the ubiquitination can also occur in the absence of RNF20 and RNF40

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EEF1D RNF40
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
2634942

Curated By

  • BioGRID