BAIT

PARP1

5830444G22Rik, AI893648, ARTD1, Adprp, Adprt1, C80510, PARP, PPOL, parp-1, sPARP-1
poly (ADP-ribose) polymerase family, member 1
Mus musculus
PREY

WWP2

1300010O06Rik, AA690238, AIP2, AW554328
WW domain containing E3 ubiquitin protein ligase 2
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.

Zhang N, Zhang Y, Qian H, Wu S, Cao L, Sun Y

The elevated expression of poly(ADP-ribose) polymerase-1 (PARP1) and increased PARP1 activity, namely, poly(ADP-ribosyl)ation (PARylation), have been observed in cardiac remodeling, leading to extreme energy consumption and myocardial damage. However, the mechanisms underlying the regulation of PARP1 require further study. WWP2, a HECT-type E3 ubiquitin ligase, is highly expressed in the heart, but its function there is largely unknown. Here, we ... [more]

Cell Death Differ Dec. 01, 2019; 27(9);2605-2619 [Pubmed: 32139900]

Throughput

  • Low Throughput

Curated By

  • BioGRID