An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rab3 interacting molecule 3 mutations associated with autism alter regulation of voltage-dependent Ca²? channels.

Takada Y, Hirano M, Kiyonaka S, Ueda Y, Yamaguchi K, Nakahara K, Mori MX, Mori Y

Autism is a neurodevelopmental psychiatric disorder characterized by impaired reciprocal social interaction, disrupted communication, and restricted and stereotyped patterns of interests. Autism is known to have a strong genetic component. Although mutations in several genes account for only a small proportion of individuals with autism, they provide insight into potential biological mechanisms that underlie autism, such as dysfunction in Ca(2+) ... [more]

Cell Calcium Sep. 01, 2015; 58(3);296-306 [Pubmed: 26142343]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RIMS3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ion channel binding [IDA]

Gene Ontology Cellular Component

CACNB4