An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SMOC1 is a tenascin-C interacting protein over-expressed in brain tumors.

Brellier F, Ruggiero S, Zwolanek D, Martina E, Hess D, Brown-Luedi M, Hartmann U, Koch M, Merlo A, Lino M, Chiquet-Ehrismann R

Tenascin-C is an extracellular matrix protein over-expressed in a large variety of cancers. In the present study, we aimed at identifying new interactors of tenascin-C by purifying secreted proteins on a tenascin-C affinity column. Analysis of eluates by mass spectrometry revealed phosphoglycerate kinase 1, clusterin, fibronectin, SPARC-related modular calcium-binding protein 1 (SMOC1) and nidogen-2 as potential interactors of tenascin-C. The ... [more]

Matrix Biol Apr. 01, 2011; 30(3);225-33 [Pubmed: 21349332]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TNC

Gene Ontology Biological Process

Gene Ontology Molecular Function

syndecan binding [IDA]

Gene Ontology Cellular Component

NID2