Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation.

Hildebrandt A, Brueggemann M, Rueckle C, Boerner S, Heidelberger JB, Busch A, Haenel H, Voigt A, Moeckel MM, Ebersberger S, Scholz A, Dold A, Schmid T, Ebersberger I, Roignant JY, Zarnack K, Koenig J, Beli P

Cells have evolved quality control mechanisms to ensure protein homeostasis by detecting and degrading aberrant mRNAs and proteins. A common source of aberrant mRNAs is premature polyadenylation, which can result in non-functional protein products. Translating ribosomes that encounter poly(A) sequences are terminally stalled, followed by ribosome recycling and decay of the truncated nascent polypeptide via ribosome-associated quality control.Here, we demonstrate ... [more]

Genome Biol. Dec. 22, 2018; 20(1);216 [Pubmed: 31640799]

Throughput

  • High Throughput

Additional Notes

  • assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MKRN1 RPL3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID