Publication

The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation.

Hildebrandt A, Brueggemann M, Rueckle C, Boerner S, Heidelberger JB, Busch A, Haenel H, Voigt A, Moeckel MM, Ebersberger S, Scholz A, Dold A, Schmid T, Ebersberger I, Roignant JY, Zarnack K, Koenig J, Beli P

Cells have evolved quality control mechanisms to ensure protein homeostasis by detecting and degrading aberrant mRNAs and proteins. A common source of aberrant mRNAs is premature polyadenylation, which can result in non-functional protein products. Translating ribosomes that encounter poly(A) sequences are terminally stalled, followed by ribosome recycling and decay of the truncated nascent polypeptide via ribosome-associated quality control.Here, we demonstrate ... [more]

Throughput

• High Throughput

Additional Notes

• assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)

Curated By

• BioGRID