Biochemical Activity (Ubiquitination)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation.

Hildebrandt A, Brueggemann M, Rueckle C, Boerner S, Heidelberger JB, Busch A, Haenel H, Voigt A, Moeckel MM, Ebersberger S, Scholz A, Dold A, Schmid T, Ebersberger I, Roignant JY, Zarnack K, Koenig J, Beli P

Cells have evolved quality control mechanisms to ensure protein homeostasis by detecting and degrading aberrant mRNAs and proteins. A common source of aberrant mRNAs is premature polyadenylation, which can result in non-functional protein products. Translating ribosomes that encounter poly(A) sequences are terminally stalled, followed by ribosome recycling and decay of the truncated nascent polypeptide via ribosome-associated quality control.Here, we demonstrate ... [more]

Genome Biol. Dec. 22, 2018; 20(1);216 [Pubmed: 31640799]

Throughput

  • Low Throughput

Additional Notes

  • in vitro ubiquitination with UBE1 as E1, UBC5a as E2, MKRN1 as E3 and the indicated hit protein as substrate

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MKRN1 RPS10
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
2907576

Curated By

  • BioGRID