An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

?2?-1 Is Essential for Sympathetic Output and NMDA Receptor Activity Potentiated by Angiotensin II in the Hypothalamus.

Ma H, Chen SR, Chen H, Li L, Li DP, Zhou JJ, Pan HL

Both the sympathetic nervous system and the renin-angiotensin system are critically involved in hypertension development. Although angiotensin II (Ang II) stimulates hypothalamic paraventricular nucleus (PVN) neurons to increase sympathetic vasomotor tone, the molecular mechanism mediating this action remains unclear. The glutamate NMDAR in the PVN controls sympathetic outflow in hypertension. In this study, we determined the interaction between ?2?-1 (encoded ... [more]

J Neurosci Dec. 11, 2017; 38(28);6388-6398 [Pubmed: 29921713]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GRIN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

N-methyl-D-aspartate selective glutamate receptor activity [IDA]calcium channel activity [IDA]calcium ion binding [ISS]calmodulin binding [ISS]extracellular-glutamate-gated ion channel activity [IBA]glutamate binding [IDA]glycine binding [IDA, IMP]neurotransmitter binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

CACNA2D1