USP8
Gene Ontology Biological Process
- cell proliferation [TAS]
- endosome organization [IMP]
- mitotic cytokinesis [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [IDA]
- protein K63-linked deubiquitination [IDA]
- protein deubiquitination [IMP]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NOTCH1
Gene Ontology Biological Process
- Notch receptor processing [TAS]
- Notch signaling involved in heart development [IMP]
- Notch signaling pathway [IMP, TAS]
- aortic valve morphogenesis [IMP]
- arterial endothelial cell differentiation [ISS]
- atrioventricular valve morphogenesis [ISS]
- cardiac atrium morphogenesis [ISS]
- cardiac chamber formation [ISS]
- cardiac epithelial to mesenchymal transition [ISS]
- cardiac left ventricle morphogenesis [ISS]
- cardiac muscle tissue morphogenesis [ISS]
- cardiac right atrium morphogenesis [ISS]
- cardiac septum morphogenesis [ISS]
- cardiac vascular smooth muscle cell development [ISS]
- cardiac ventricle morphogenesis [ISS]
- cell migration involved in endocardial cushion formation [ISS]
- cellular response to follicle-stimulating hormone stimulus [IDA]
- cellular response to vascular endothelial growth factor stimulus [IDA]
- cilium morphogenesis [ISS]
- coronary artery morphogenesis [ISS]
- coronary vein morphogenesis [ISS]
- determination of left/right symmetry [ISS]
- endocardial cell differentiation [ISS]
- endocardial cushion morphogenesis [ISS]
- endocardium development [ISS]
- endocardium morphogenesis [ISS]
- epithelial to mesenchymal transition [ISS]
- epithelial to mesenchymal transition involved in endocardial cushion formation [ISS]
- gene expression [TAS]
- growth involved in heart morphogenesis [ISS]
- heart development [IMP]
- heart looping [ISS]
- heart trabecula morphogenesis [ISS]
- immune response [NAS]
- mesenchymal cell development [ISS]
- mitral valve formation [IMP]
- negative regulation of BMP signaling pathway [ISS]
- negative regulation of anoikis [IMP]
- negative regulation of catalytic activity [ISS]
- negative regulation of cell migration involved in sprouting angiogenesis [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of cell-substrate adhesion [IDA]
- negative regulation of endothelial cell chemotaxis [IDA]
- negative regulation of glial cell proliferation [ISS]
- negative regulation of myoblast differentiation [IMP]
- negative regulation of myotube differentiation [ISS]
- negative regulation of neurogenesis [ISS]
- negative regulation of oligodendrocyte differentiation [ISS]
- negative regulation of ossification [ISS]
- negative regulation of osteoblast differentiation [ISS]
- negative regulation of pro-B cell differentiation [ISS]
- negative regulation of stem cell differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- negative regulation of transcription, DNA-templated [ISS]
- neuronal stem cell maintenance [IEP]
- pericardium morphogenesis [ISS]
- positive regulation of BMP signaling pathway [ISS]
- positive regulation of JAK-STAT cascade [ISS]
- positive regulation of astrocyte differentiation [ISS]
- positive regulation of cardiac muscle cell proliferation [ISS]
- positive regulation of cell migration [ISS]
- positive regulation of cell proliferation [IDA, IMP]
- positive regulation of epithelial to mesenchymal transition [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- positive regulation of transcription from RNA polymerase II promoter in response to hypoxia [ISS]
- positive regulation of transcription of Notch receptor target [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- pulmonary valve morphogenesis [IMP]
- regulation of extracellular matrix assembly [ISS]
- regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [ISS]
- regulation of transcription, DNA-templated [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- tube formation [IMP]
- vasculogenesis involved in coronary vascular morphogenesis [ISS]
- venous endothelial cell differentiation [ISS]
- ventricular septum morphogenesis [IMP]
- ventricular trabecula myocardium morphogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Deubiquitylation and stabilization of Notch1 intracellular domain by ubiquitin-specific protease 8 enhance tumorigenesis in breast cancer.
Notch, an essential factor in tissue development and homoeostasis, has been reported to play an oncogenic function in a variety of cancers. Here, we report ubiquitin-specific protease 8 (USP8) as a novel deubiquitylase of Notch1 intracellular domain (NICD). USP8 specifically stabilizes and deubiquitylates NICD through a direct interaction. The inhibition of USP8 downregulated the Notch signalling pathway via NICD destabilization, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NOTCH1 USP8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP8 NOTCH1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NOTCH1 USP8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID