BAIT

EDE1

BUD15, YBL047C
Scaffold protein involved in the formation of early endocytic sites; putative regulator of cytokinesis; homo-oligomerization is required for localization to and organization of endocytic sites; has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death; homolog of mammalian Eps15
UPS Project
GO Process (4)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

VPS27

DID7, GRD11, SSV17, VPL23, VPT27, ESCRT-0 subunit protein VPS27, L000002790, YNR006W
Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
UPS Project
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits.

Wilfling F, Lee CW, Erdmann PS, Zheng Y, Sherpa D, Jentsch S, Pfander B, Schulman BA, Baumeister W

Autophagy eliminates cytoplasmic content selected by autophagy receptors, which link cargo to the membrane-bound autophagosomal ubiquitin-like protein Atg8/LC3. Here, we report a selective autophagy pathway for protein condensates formed by endocytic proteins in yeast. In this pathway, the endocytic protein Ede1 functions as a selective autophagy receptor. Distinct domains within Ede1 bind Atg8 and mediate phase separation into condensates. Both ... [more]

Mol Cell Dec. 03, 2019; 80(5);764-778.e7 [Pubmed: 33207182]

Throughput

  • High Throughput

Ontology Terms

  • protein/peptide accumulation (APO:0000149)

Additional Notes

  • An EGFP-Ede1 overexpression strain was crossed against a deletion library of non-essential yeast genes.
  • This experiment identified 100 proteins (Z score > 1.5) defective in END degradation as indicated by Ede1-dependent endocytic protein deposits (ENDs).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EDE1 VPS27
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-2.8402BioGRID
514941

Curated By

  • BioGRID