SLC6A1
Gene Ontology Biological Process
- gamma-aminobutyric acid import [IDA]
- learning [IMP]
- negative regulation of synaptic transmission, GABAergic [IMP]
- positive regulation of gamma-aminobutyric acid secretion [IMP]
- protein homooligomerization [IDA]
- response to calcium ion [IEP]
- response to cocaine [IEP]
- response to estradiol [IEP]
- response to lead ion [IEP]
- response to organic cyclic compound [IEP]
- response to organic substance [IMP]
- response to organonitrogen compound [IEP]
- response to purine-containing compound [IMP]
- response to sucrose [IEP]
- response to toxic substance [IEP, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARFGAP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Sec24- and ARFGAP1-dependent trafficking of GABA transporter-1 is a prerequisite for correct axonal targeting.
The GABA transporter-1 (GAT1) is a prototypical protein of the synaptic specialization. Export of GAT1 from the endoplasmic reticulum (ER) is contingent on its interaction with the COPII (coatomer protein-II) coat subunit Sec24D. Here we show that silencing all four Sec24 isoforms strongly inhibits transport of GAT1 to the cell surface. In contrast, transport of GAT1-RL/AS, a mutant that is ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID