RAF1
Gene Ontology Biological Process
- MAPK cascade [ISO]
- activation of MAPKK activity [ISO]
- cell differentiation [IGI]
- death-inducing signaling complex assembly [IMP]
- intermediate filament cytoskeleton organization [IMP]
- intracellular signal transduction [TAS]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IGI, IMP]
- negative regulation of protein complex assembly [ISO]
- neurotrophin TRK receptor signaling pathway [IMP]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- protein phosphorylation [ISO, TAS]
- response to hypoxia [ISO]
- response to muscle stretch [IMP]
- somatic stem cell maintenance [IGI]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase kinase activity [ISO]
- Ras GTPase binding [IPI]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [ISO]
- ATP binding [ISO]
- MAP kinase kinase kinase activity [ISO]
- Ras GTPase binding [IPI]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [ISO]
Gene Ontology Cellular Component
HRAS
Gene Ontology Biological Process
- Ras protein signal transduction [ISO, TAS]
- apoptotic process [ISO]
- cell aging [IDA]
- cell cycle arrest [ISO]
- cell proliferation [IPI]
- cellular senescence [ISO]
- endocytosis [IDA]
- intrinsic apoptotic signaling pathway [IGI, IMP]
- mitotic cell cycle checkpoint [ISO]
- negative regulation of Rho GTPase activity [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of neuron apoptotic process [IDA, IGI]
- positive regulation of DNA replication [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of JNK cascade [ISO]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of Rac GTPase activity [ISO]
- positive regulation of Ras protein signal transduction [ISO]
- positive regulation of actin cytoskeleton reorganization [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [IDA, ISO]
- positive regulation of epithelial cell proliferation [ISO]
- positive regulation of gene expression [IDA]
- positive regulation of miRNA metabolic process [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of ruffle assembly [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of wound healing [ISO]
- protein heterooligomerization [ISO]
- regulation of long-term neuronal synaptic plasticity [IMP]
- small GTPase mediated signal transduction [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Identification of signalling proteins interacting with B-Raf in the yeast two-hybrid system.
Recent studies suggested the existence of Ras/B-Raf/ MEK-1 complexes and a critical role for B-Raf in regulating the MAP kinase/ERKs signalling pathway. We report, here, that both Ras and MEK-1 proteins interact physically with B-Raf proteins in the yeast two-hybrid system. In addition, by screening a mouse brain cDNA library, we isolated additional B-Raf interacting proteins. These include three members ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAF1 HRAS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAF1 HRAS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAF1 HRAS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAF1 HRAS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAF1 HRAS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAF1 HRAS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAF1 HRAS | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1416798 | |
| HRAS RAF1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| HRAS RAF1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID