BAIT
RAF1
6430402F14Rik, AA990557, BB129353, Craf1, D830050J10Rik, Raf-1, c-Raf, v-Raf
v-raf-leukemia viral oncogene 1
GO Process (16)
GO Function (9)
GO Component (5)
Gene Ontology Biological Process
- MAPK cascade [ISO]
- activation of MAPKK activity [ISO]
- cell differentiation [IGI]
- death-inducing signaling complex assembly [IMP]
- intermediate filament cytoskeleton organization [IMP]
- intracellular signal transduction [TAS]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IGI, IMP]
- negative regulation of protein complex assembly [ISO]
- neurotrophin TRK receptor signaling pathway [IMP]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- protein phosphorylation [ISO, TAS]
- response to hypoxia [ISO]
- response to muscle stretch [IMP]
- somatic stem cell maintenance [IGI]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase kinase activity [ISO]
- Ras GTPase binding [IPI]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [ISO]
- ATP binding [ISO]
- MAP kinase kinase kinase activity [ISO]
- Ras GTPase binding [IPI]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
MAP2K2
AA589381, MEK2, MK2, Prkmk2
mitogen-activated protein kinase kinase 2
GO Process (9)
GO Function (8)
GO Component (15)
Gene Ontology Biological Process
- MAPK cascade [ISO]
- activation of MAPK activity [ISO, TAS]
- peptidyl-serine autophosphorylation [ISO]
- positive regulation of cell motility [IDA]
- positive regulation of protein serine/threonine kinase activity [ISO]
- protein phosphorylation [ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of stress-activated MAPK cascade [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO, TAS]
- cell cortex [ISO]
- cell-cell junction [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic side of plasma membrane [ISO]
- cytosol [ISO, TAS]
- early endosome [TAS]
- endoplasmic reticulum [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule [ISO]
- mitochondrion [TAS]
- nucleus [TAS]
- perinuclear region of cytoplasm [ISO]
- peroxisomal membrane [ISO]
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Identification of signalling proteins interacting with B-Raf in the yeast two-hybrid system.
Recent studies suggested the existence of Ras/B-Raf/ MEK-1 complexes and a critical role for B-Raf in regulating the MAP kinase/ERKs signalling pathway. We report, here, that both Ras and MEK-1 proteins interact physically with B-Raf proteins in the yeast two-hybrid system. In addition, by screening a mouse brain cDNA library, we isolated additional B-Raf interacting proteins. These include three members ... [more]
Oncogene May. 16, 1996; 12(10);2213-21 [Pubmed: 8668348]
Throughput
- Low Throughput
Curated By
- BioGRID