PREY

PRKACA

PKCD, Pkaca
protein kinase, cAMP dependent, catalytic, alpha
GO Process (20)
GO Function (10)
GO Component (16)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Protein kinase A-induced phosphorylation at the Thr154 affects stability of DJ-1.

Ko YU, Kim SJ, Lee J, Song MY, Park KS, Park JB, Cho HS, Oh YJ

Most cases of Parkinson's disease (PD) are sporadic, but genetic variations have been discovered in PD patients. PARK7/DJ-1 is a known cause of early-onset autosomal-recessive PD and is implicated in neuroprotection against oxidative stress. Although several post-translational modifications of DJ-1 have been proposed, phospho-modification of DJ-1 and its functional consequences have been less studied.Putative phosphorylation sites of DJ-1 were determined ... [more]

Parkinsonism Relat Disord Dec. 01, 2018; 66();143-150 [Pubmed: 31353306]

Throughput

  • Low Throughput

Curated By

  • BioGRID