Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Temporal-spatial expression and novel biochemical properties of the memory-related protein KIBRA.

Johannsen S, Duning K, Pavenstaedt H, Kremerskothen J, Boeckers TM

The ability of the mammalian brain to store and recall information is based on synaptic plasticity due to constant remodeling of synaptic contacts. Although various classes of proteins such as neurotransmitter receptors, cytoskeletal components and protein kinases were already identified as modulators of memory formation, their specific interactions and crosstalks are still poorly understood. Genetic variants of the scaffolding protein ... [more]

Neuroscience Sep. 09, 2008; 155(4);1165-73 [Pubmed: 18672031]

Throughput

  • Low Throughput

Curated By

  • BioGRID