BAIT

TIF3B1

ARABIDOPSIS THALIANA TRANSLATION INITIATION FACTOR 3B1, ATEIF3B-1, ATTIF3B1, EIF3B, EIF3B-1, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B, EUKARYOTIC TRANSLATION INITIATION FACTOR 3B1, T1G16.2, translation initiation factor 3B1, AT5G27640
translation initiation factor 3 subunit B
GO Process (0)
GO Function (2)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

EIF3C

ATEIF3C-1, ATTIF3C1, EIF3C-1, EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 8, TIF3C1, eukaryotic translation initiation factor 3C, AT3G56150
eukaryotic translation initiation factor 3 subunit C
GO Process (0)
GO Function (2)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

  • 0.989119048 [CF-MS score]

Throughput

  • High Throughput

Additional Notes

  • Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
  • Interactor A: Orthogroup ENOG411DQH3, Annotation: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex (By similarity)
  • Interactor B: Orthogroup ENOG411DRJ6, Annotation: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex (By similarity)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TIF3B1 EIF3C
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
EIF3C TIF3B1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID