BAIT

AT1G45000

F27F5.8, F27F5_8
AAA-type ATPase family protein
GO Process (0)
GO Function (1)
GO Component (7)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

RPT5B

26S proteasome AAA-ATPase subunit RPT5B, F7G19.2, F7G19_2, AT1G09100
26S proteasome AAA-ATPase subunit RPT5B
GO Process (4)
GO Function (2)
GO Component (4)
Arabidopsis thaliana (Columbia)

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

  • 0.9762278 [CF-MS score]

Throughput

  • High Throughput

Additional Notes

  • Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
  • Interactor A: Orthogroup ENOG411DVFS, Annotation: 26S protease regulatory subunit
  • Interactor B: Orthogroup ENOG411DXX0, Annotation: 26S protease regulatory subunit 6A

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPT5B AT1G45000
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID