Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

0.916508658 [CF-MS score]

Throughput

High Throughput

Additional Notes

Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
Interactor A: Orthogroup ENOG411DWD0, Annotation: 26s proteasome regulatory subunit
Interactor B: Orthogroup ENOG411DY5N, Annotation: 26S proteasome non-atpase regulatory subunit

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPN1A EMB2719	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Van Leene J (2010)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RPN1A

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin binding [IDA]

Gene Ontology Cellular Component

EMB2719