BAIT

VPS35B

T4O12.9, T4O12_9, VPS35 homolog B, AT1G75850
vacuolar protein sorting-associated protein 35B
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Arabidopsis thaliana (Columbia)
PREY

VPS26B

T29A15.180, T29A15_180, vacuolar protein sorting 26B, AT4G27690
vacuolar protein sorting-associated protein 26B
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

  • 0.905352453 [CF-MS score]

Throughput

  • High Throughput

Additional Notes

  • Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
  • Interactor A: Orthogroup ENOG411DT6Y, Annotation: Vacuolar protein sorting-associated protein
  • Interactor B: Orthogroup ENOG411DWDF, Annotation: Vacuolar protein sorting-associated protein

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VPS35B VPS26B
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
VPS35B VPS26B
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID