Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

0.551638709 [CF-MS score]

Throughput

High Throughput

Additional Notes

Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
Interactor A: Orthogroup ENOG411DT8H, Annotation: eukaryotic initiation factor
Interactor B: Orthogroup ENOG411E36A, Annotation: RNA-binding protein

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
Y14 EIF4A1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Koroleva OA (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EIF4A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent helicase activity [ISS]protein binding [IPI]translation initiation factor activity [ISS]

Gene Ontology Cellular Component

Y14