BAIT

GAPA

GAPA-1, GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE A SUBUNIT, GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE A SUBUNIT 1, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE A SUBUNIT, AT3G26650
glyceraldehyde-3-phosphate dehydrogenase A subunit
GO Process (5)
GO Function (1)
GO Component (7)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

PRK

T12O21.4, T12O21_4, phosphoribulokinase, AT1G32060
phosphoribulokinase
Arabidopsis thaliana (Columbia)

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

  • 0.52323051 [CF-MS score]

Throughput

  • High Throughput

Additional Notes

  • Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
  • Interactor A: Orthogroup ENOG411DTEA, Annotation: glyceraldehyde-3-phosphate dehydrogenase
  • Interactor B: Orthogroup ENOG411EKD0, Annotation: Phosphoribulokinase

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GAPA PRK
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID