Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome.

Dayebgadoh G, Sardiu ME, Florens L, Washburn MP

A hub protein in protein interaction networks will typically have a large number of diverse interactions. Determining the core interactions and the function of such a hub protein remains a significant challenge in the study of networks. Proteins with WD40 repeats represent a large class of proteins that can be hub proteins. WDR76 is a poorly characterized WD40 repeat protein ... [more]

J Proteome Res Dec. 06, 2018; 18(9);3479-3491 [Pubmed: 31353912]

Throughput

  • High Throughput

Additional Notes

  • chromatin-enriched nuclear extract
  • whole cell extract

Curated By

  • BioGRID