GLI1
Gene Ontology Biological Process
- cerebellar cortex morphogenesis [IGI]
- dorsal/ventral pattern formation [IGI]
- epidermal cell differentiation [ISO]
- lung development [IGI]
- negative regulation of canonical Wnt signaling pathway [ISO]
- notochord regression [IGI]
- osteoblast differentiation [ISO]
- pituitary gland development [IGI]
- positive regulation of DNA replication [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of smoothened signaling pathway [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISO]
- proximal/distal pattern formation [IGI]
- signal transduction [TAS]
- smoothened signaling pathway [IDA, IGI, ISO]
- smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation [IGI]
- spermatogenesis [IDA]
- transcription from RNA polymerase II promoter [IDA]
- ventral midline development [IGI]
Gene Ontology Molecular Function
SUFU
Gene Ontology Biological Process
- cytoplasmic sequestering of transcription factor [IGI]
- determination of left/right symmetry [IMP]
- heart looping [IMP]
- negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IGI]
- negative regulation of smoothened signaling pathway [IDA, IMP]
- negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- neural tube closure [IMP]
- skin development [IMP]
- smoothened signaling pathway involved in spinal cord motor neuron cell fate specification [IMP]
- smoothened signaling pathway involved in ventral spinal cord interneuron specification [IMP]
- spinal cord dorsal/ventral patterning [IMP]
Gene Ontology Molecular Function
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
Overlapping binding sites for importin ?1 and suppressor of fused (SuFu) on glioma-associated oncogene homologue 1 (Gli1) regulate its nuclear localization.
A key factor in oncogenesis is the transport into the nucleus of oncogenic signalling molecules, such as Gli1 (glioma-associated oncogene homologue 1), the central transcriptional activator in the Hedgehog signalling pathway. Little is known, however, how factors such as Gli are transported into the nucleus and how this may be regulated by interaction with other cellular factors, such as the ... [more]
Throughput
- Low Throughput
Additional Notes
- amplified luminescent proximity homogeneous assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GLI1 SUFU | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| SUFU GLI1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SUFU GLI1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| GLI1 SUFU | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID