BAIT
GLI1
AV235269, Zfp-5, Zfp5
GLI-Kruppel family member GLI1
GO Process (20)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- cerebellar cortex morphogenesis [IGI]
- dorsal/ventral pattern formation [IGI]
- epidermal cell differentiation [ISO]
- lung development [IGI]
- negative regulation of canonical Wnt signaling pathway [ISO]
- notochord regression [IGI]
- osteoblast differentiation [ISO]
- pituitary gland development [IGI]
- positive regulation of DNA replication [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of smoothened signaling pathway [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISO]
- proximal/distal pattern formation [IGI]
- signal transduction [TAS]
- smoothened signaling pathway [IDA, IGI, ISO]
- smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation [IGI]
- spermatogenesis [IDA]
- transcription from RNA polymerase II promoter [IDA]
- ventral midline development [IGI]
Gene Ontology Molecular Function
Mus musculus
PREY
KPNB1
AA409963, IPOB, Impnb, RP23-81D2.3
karyopherin (importin) beta 1
GO Process (2)
GO Function (5)
GO Component (10)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
Overlapping binding sites for importin ?1 and suppressor of fused (SuFu) on glioma-associated oncogene homologue 1 (Gli1) regulate its nuclear localization.
A key factor in oncogenesis is the transport into the nucleus of oncogenic signalling molecules, such as Gli1 (glioma-associated oncogene homologue 1), the central transcriptional activator in the Hedgehog signalling pathway. Little is known, however, how factors such as Gli are transported into the nucleus and how this may be regulated by interaction with other cellular factors, such as the ... [more]
Biochem J Aug. 01, 2014; 461(3);469-76 [Pubmed: 24854174]
Throughput
- Low Throughput
Additional Notes
- amplified luminescent proximity homogeneous assay
Curated By
- BioGRID