An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Partners of wild type Grb7 and a mutant lacking its calmodulin-binding domain.

Garcia-Palmero I, Shah N, Ali NA, Daly RJ, Wilce JA, Villalobo A

Growth factor receptor bound protein 7 (Grb7) is a mammalian adaptor protein participating in signaling pathways implicated in cell migration, metastatic invasion, cell proliferation and tumor-associated angiogenesis. We expressed tagged versions of wild type Grb7 and the mutant Grb7?, lacking its calmodulin-binding domain (CaM-BD), in human embryonic kidney (HEK) 293 cells and rat glioma C6 cells to identify novel binding partners ... [more]

Arch Biochem Biophys Dec. 15, 2019; 687();108386 [Pubmed: 32360748]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

GRB7

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3/SH2 adaptor activity [TAS]identical protein binding [IPI]phosphatidylinositol binding [IDA]protein binding [IPI]protein kinase binding [IDA]

Gene Ontology Cellular Component

CCT7