GRB7
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSPA8
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- chaperone mediated protein folding requiring cofactor [IDA, ISO]
- chaperone-mediated protein folding [IDA]
- clathrin coat disassembly [ISO]
- negative regulation of fibril organization [ISO]
- negative regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation of mRNA splicing, via spliceosome [ISO]
- protein folding [ISO]
- protein refolding [ISO]
- regulation of cell cycle [ISO]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
Gene Ontology Cellular Component
- Prp19 complex [ISO, ISS]
- blood microparticle [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [IDA, ISO]
- focal adhesion [ISO]
- intracellular [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleus [ISO, ISS]
- protein complex [IDA]
- ribonucleoprotein complex [ISO, ISS]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
- ubiquitin ligase complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Partners of wild type Grb7 and a mutant lacking its calmodulin-binding domain.
Growth factor receptor bound protein 7 (Grb7) is a mammalian adaptor protein participating in signaling pathways implicated in cell migration, metastatic invasion, cell proliferation and tumor-associated angiogenesis. We expressed tagged versions of wild type Grb7 and the mutant Grb7?, lacking its calmodulin-binding domain (CaM-BD), in human embryonic kidney (HEK) 293 cells and rat glioma C6 cells to identify novel binding partners ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GRB7 HSPA8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID