Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Nano-LC based proteomic approach identifies that E6AP interacts with ENO1 and targets it for degradation in breast cancer cells.

Mishra M, Sharma A, Thacker G, Trivedi AK

E6AP (E6 associated protein) is a HECT domain containing protein having dual E3 ligase and ER? coactivation activity in breast cancer cells. Although E6AP is known to possess antitumorigenic activity, the underlying molecular mechanism is poorly understood. In the present study, we applied nano-LC based proteomics approach to identify E6AP-interacting proteins where we performed GST-pull down using GST-E6AP from whole ... [more]

IUBMB Life Dec. 01, 2018; 71(12);1896-1905 [Pubmed: 31329371]

Throughput

  • High Throughput

Curated By

  • BioGRID