An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

NCBP1 promotes the development of lung adenocarcinoma through up-regulation of CUL4B.

Zhang H, Wang A, Tan Y, Wang S, Ma Q, Chen X, He Z

Lung cancer is the most frequent cancer type and is the leading cause of tumour-associated deaths worldwide. Nuclear cap-binding protein 1 (NCBP1) is necessary for capped RNA processing and intracellular localization. It has been reported that silencing of NCBP1 resulted in cell growth reduction in HeLa cells. Nevertheless, its clinical significance and underlying molecular mechanisms in non-small-cell lung cancer remain ... [more]

J Cell Mol Med Dec. 01, 2018; 23(10);6965-6977 [Pubmed: 31448526]

Throughput

Low Throughput

Additional Notes

protein corresponding to the bait genes NCBP3 was immunoprecipitated from cell lysates and associated RNA corresponding to the hit genes NCBP1 and NCBP3, but not NCBP2, were detected by qRT-PCR (quantitative reverse transcriptase polymerase chain reaction)

Curated By

BioGRID

Interaction Summary

C17ORF85

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]