An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Thioredoxin interacting protein (Txnip) forms redox sensitive high molecular weight nucleoprotein complexes.

Hirata CL, Ito S, Masutani H

Thioredoxin interacting protein (Txnip) is an ?-arrestin protein that regulates pleiotropic biological responses. Txnip acts as a cancer suppressor and is a critical regulator of energy metabolism. To investigate molecular mechanisms involving Txnip, we searched for its protein binding partners using tandem affinity purification and proteomics analyses and identified several viable candidates, including HSP90, HSP70, and Prp31. We showed, by ... [more]

Arch Biochem Biophys Dec. 30, 2018; 677();108159 [Pubmed: 31669268]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TXNIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

HSPA4