TRIM71
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISS]
- fibroblast growth factor receptor signaling pathway [ISS]
- miRNA metabolic process [ISS]
- negative regulation of translation involved in gene silencing by miRNA [ISS]
- neural tube development [ISS]
- protein autoubiquitination [ISS]
- regulation of gene silencing by miRNA [ISS]
- regulation of neural precursor cell proliferation [ISS]
- stem cell proliferation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDKN1A
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IDA, TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- G1/S transition of mitotic cell cycle [IDA, TAS]
- G2/M transition of mitotic cell cycle [IMP]
- Ras protein signal transduction [IEP]
- cell cycle arrest [IDA, IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to extracellular stimulus [IMP]
- cellular response to ionizing radiation [IMP]
- cellular senescence [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- mitotic cell cycle [TAS]
- negative regulation of G1/S transition of mitotic cell cycle [IGI]
- negative regulation of cell growth [IDA]
- negative regulation of cell proliferation [IDA, IMP]
- negative regulation of phosphorylation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of fibroblast proliferation [IMP]
- positive regulation of protein kinase activity [IDA]
- positive regulation of reactive oxygen species metabolic process [IMP]
- protein phosphorylation [IDA]
- regulation of cyclin-dependent protein serine/threonine kinase activity [TAS]
- stress-induced premature senescence [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
The mRNA repressor TRIM71 cooperates with Nonsense-Mediated Decay factors to destabilize the mRNA of CDKN1A/p21.
Nonsense-mediated decay (NMD) plays a fundamental role in the degradation of premature termination codon (PTC)-containing transcripts, but also regulates the expression of functional transcripts lacking PTCs, although such 'non-canonical' functions remain ill-defined and require the identification of factors targeting specific mRNAs to the NMD machinery. Our work identifies the stem cell-specific mRNA repressor protein TRIM71 as one of these factors. ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using RIP (RNA immunoprecipitation) in which the bait protein TRIM71 was immunoprecipitated and RNA corresponding to the hit gene CDKN1A was detected by qPCR (quantitative polymerase chain reaction)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CDKN1A TRIM71 | Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro. | Low | - | BioGRID | 2933624 |
Curated By
- BioGRID