BAIT
UPF1
HUPF1, NORF1, RENT1, pNORF1, smg-2
UPF1 regulator of nonsense transcripts homolog (yeast)
GO Process (11)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA repair [IDA]
- DNA replication [IMP]
- RNA metabolic process [TAS]
- gene expression [TAS]
- histone mRNA catabolic process [IMP]
- mRNA export from nucleus [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IDA, IMP, NAS, TAS]
- regulation of translational termination [IMP, NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATF3
RP11-338C15.1
activating transcription factor 3
GO Process (4)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- identical protein binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription corepressor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- identical protein binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription corepressor activity [TAS]
Homo sapiens
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
The mRNA repressor TRIM71 cooperates with Nonsense-Mediated Decay factors to destabilize the mRNA of CDKN1A/p21.
Nonsense-mediated decay (NMD) plays a fundamental role in the degradation of premature termination codon (PTC)-containing transcripts, but also regulates the expression of functional transcripts lacking PTCs, although such 'non-canonical' functions remain ill-defined and require the identification of factors targeting specific mRNAs to the NMD machinery. Our work identifies the stem cell-specific mRNA repressor protein TRIM71 as one of these factors. ... [more]
Nucleic Acids Res Dec. 16, 2018; 47(22);11861-11879 [Pubmed: 31732746]
Throughput
- Low Throughput
Additional Notes
- assayed using RIP (RNA immunoprecipitation) in which the bait protein UPF1 was immunoprecipitated and RNA corresponding to the hit genes indicate were detected by qPCR (quantitative polymerase chain reaction)
Curated By
- BioGRID