BAIT
UPF1
HUPF1, NORF1, RENT1, pNORF1, smg-2
UPF1 regulator of nonsense transcripts homolog (yeast)
GO Process (11)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA repair [IDA]
- DNA replication [IMP]
- RNA metabolic process [TAS]
- gene expression [TAS]
- histone mRNA catabolic process [IMP]
- mRNA export from nucleus [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IDA, IMP, NAS, TAS]
- regulation of translational termination [IMP, NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
E2F7
E2F transcription factor 7
GO Process (13)
GO Function (5)
GO Component (0)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator [IDA]
- chorionic trophoblast cell differentiation [ISS]
- hepatocyte differentiation [ISS]
- negative regulation of G1/S transition of mitotic cell cycle [ISS]
- negative regulation of cell proliferation [IMP]
- negative regulation of cytokinesis [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription involved in G1/S transition of mitotic cell cycle [IDA]
- placenta development [ISS]
- positive regulation of DNA endoreduplication [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- sprouting angiogenesis [IMP]
- trophoblast giant cell differentiation [ISS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
The mRNA repressor TRIM71 cooperates with Nonsense-Mediated Decay factors to destabilize the mRNA of CDKN1A/p21.
Nonsense-mediated decay (NMD) plays a fundamental role in the degradation of premature termination codon (PTC)-containing transcripts, but also regulates the expression of functional transcripts lacking PTCs, although such 'non-canonical' functions remain ill-defined and require the identification of factors targeting specific mRNAs to the NMD machinery. Our work identifies the stem cell-specific mRNA repressor protein TRIM71 as one of these factors. ... [more]
Nucleic Acids Res Dec. 16, 2018; 47(22);11861-11879 [Pubmed: 31732746]
Throughput
- Low Throughput
Additional Notes
- assayed using RIP (RNA immunoprecipitation) in which the bait protein UPF1 was immunoprecipitated and RNA corresponding to the hit genes indicate were detected by qPCR (quantitative polymerase chain reaction)
Curated By
- BioGRID