GAD1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CALM1
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [ISO]
- activation of adenylate cyclase activity [IDA]
- calcium-mediated signaling [IMP]
- detection of calcium ion [ISO]
- positive regulation of DNA binding [ISO]
- positive regulation of cyclic nucleotide metabolic process [ISO]
- positive regulation of cyclic-nucleotide phosphodiesterase activity [ISO]
- positive regulation of nitric-oxide synthase activity [IDA]
- positive regulation of phosphoprotein phosphatase activity [ISO]
- positive regulation of protein dephosphorylation [ISO]
- positive regulation of ryanodine-sensitive calcium-release channel activity [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of cytokinesis [ISO]
- regulation of heart rate [ISO]
- regulation of high voltage-gated calcium channel activity [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISO]
- regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- regulation of store-operated calcium channel activity [IC]
- response to amphetamine [IEP]
- response to calcium ion [ISO]
- substantia nigra development [ISO]
Gene Ontology Molecular Function- N-terminal myristoylation domain binding [ISO]
- adenylate cyclase binding [IDA]
- calcium channel regulator activity [NAS]
- calcium ion binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- enzyme regulator activity [IMP]
- ion channel binding [IDA, ISO]
- nitric-oxide synthase binding [IDA]
- nitric-oxide synthase regulator activity [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- phospholipase binding [ISO]
- protein N-terminus binding [IMP]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase activator activity [ISO]
- thioesterase binding [ISO]
- titin binding [ISO]
- type 3 metabotropic glutamate receptor binding [IPI]
- N-terminal myristoylation domain binding [ISO]
- adenylate cyclase binding [IDA]
- calcium channel regulator activity [NAS]
- calcium ion binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- enzyme regulator activity [IMP]
- ion channel binding [IDA, ISO]
- nitric-oxide synthase binding [IDA]
- nitric-oxide synthase regulator activity [IDA]
- phosphatidylinositol 3-kinase binding [IPI]
- phospholipase binding [ISO]
- protein N-terminus binding [IMP]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase activator activity [ISO]
- thioesterase binding [ISO]
- titin binding [ISO]
- type 3 metabotropic glutamate receptor binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Effect of apocalmodulin on recombinant human brain glutamic acid decarboxylase.
In this work, we report that the recombinant glutathione S-transferase (GST)-human L-glutamic acid decarboxylase (HGAD) isoforms, 65-kDa L-glutamic acid decarboxylase (GAD) (GST-HGAD65) fusion protein or free truncated HGAD65, were activated by apocalmodulin (ApoCaM) to an extent of 60%. Both truncated forms of GAD67 (tGAD67), HGAD67(Delta1-70) and HGAD67(Delta1-90), were markedly activated by ApoCaM to an extent of 141 and 85%, respectively, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID